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2% alginate solution needed by dividing the total cell number by 4 106 (d) When resuspending the chondrocytes in the 12% alginate solution, do not add more than 5 ml alginate solution initially Mix well and then add the remaining alginate solution needed to obtain the desired chondrocyte concentration (e) Add 50 ml sterile 102 mM CaCl2 solution to a sterile sample cup (f) Add 7 ml chondrocyte-alginate mixture to 50 ml sterile 102 mM CaCl2 solution This step is particularly important for human chondrocyte preparations Up to 12 ml chondrocyte-alginate mixture may be added to 50 ml sterile 102 mM CaCl2 solution: i) The CaCl2 solution should be stirred constantly with a sterile stirrer bar ii) Use either a sterile syringe that has a sterile 22-gauge needle attached or a disposable sterile pipette (5 ml or 10 ml) attached to a peristaltic pump. rdlc gs1 128 Packages matching GS1-128 - NuGet Gallery
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